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construct encoding memerald sec61b c1  (Addgene inc)


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    Addgene inc construct encoding memerald sec61b c1
    Construct Encoding Memerald Sec61b C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/construct+encoding+memerald+sec61b+c1/mEmerald-Sec61b-C1+(Plasmid+%2390992)/pmc09035751-46-13-24
    Average 93 stars, based on 29 article reviews
    construct encoding memerald sec61b c1 - by Bioz Stars, 2026-10
    93/100 stars

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    Transfection:

    Article Title: Correlative Organelle Microscopy: fluorescence guided volume electron microscopy of intracellular processes
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 , which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 hours. .. Transfections were performed using Effectene transfection reagent (Qiagen) according to manufacturer’s instructions.

    Article Title: Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 , which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 h. Transfections were performed using Effectene transfection reagent (Qiagen) according to the manufacturer’s instructions. .. Prior to FM, cells were incubated with fiducial markers ( ) at a concentration of 1 μg/ml and cell-permeable lysosome stain SiR-Lysosome (SpiroChrome) at a concentration of 0.5 μM in complete DMEM and incubated for 3 h.

    Article Title: Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes.
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 (Nixon-Abell et al., 2016), which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 h. Transfections were performed using Effectene transfection reagent (Qiagen) according to the manufacturer’s instructions. .. Prior to FM, cells were incubated with fiducial markers (Fokkema et al., 2018) at a concentration of 1 μg/ml and cell-permeable lysosome stain SiR-Lysosome (SpiroChrome) at a concentration of 0.5 μM in complete DMEM and incubated for 3 h.

    Construct:

    Article Title: Correlative Organelle Microscopy: fluorescence guided volume electron microscopy of intracellular processes
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 , which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 hours. .. Transfections were performed using Effectene transfection reagent (Qiagen) according to manufacturer’s instructions.

    Article Title: Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 , which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 h. Transfections were performed using Effectene transfection reagent (Qiagen) according to the manufacturer’s instructions. .. Prior to FM, cells were incubated with fiducial markers ( ) at a concentration of 1 μg/ml and cell-permeable lysosome stain SiR-Lysosome (SpiroChrome) at a concentration of 0.5 μM in complete DMEM and incubated for 3 h.

    Article Title: Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes.
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 (Nixon-Abell et al., 2016), which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 h. Transfections were performed using Effectene transfection reagent (Qiagen) according to the manufacturer’s instructions. .. Prior to FM, cells were incubated with fiducial markers (Fokkema et al., 2018) at a concentration of 1 μg/ml and cell-permeable lysosome stain SiR-Lysosome (SpiroChrome) at a concentration of 0.5 μM in complete DMEM and incubated for 3 h.

    Plasmid Preparation:

    Article Title: Correlative Organelle Microscopy: fluorescence guided volume electron microscopy of intracellular processes
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 , which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 hours. .. Transfections were performed using Effectene transfection reagent (Qiagen) according to manufacturer’s instructions.

    Article Title: Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 , which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 h. Transfections were performed using Effectene transfection reagent (Qiagen) according to the manufacturer’s instructions. .. Prior to FM, cells were incubated with fiducial markers ( ) at a concentration of 1 μg/ml and cell-permeable lysosome stain SiR-Lysosome (SpiroChrome) at a concentration of 0.5 μM in complete DMEM and incubated for 3 h.

    Article Title: Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes.
    Article Snippet: .. On the next day following seeding, the cells were transiently transfected with a construct encoding mEmerald-Sec61b-C1 (Nixon-Abell et al., 2016), which was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 90992), for 16 h. Transfections were performed using Effectene transfection reagent (Qiagen) according to the manufacturer’s instructions. .. Prior to FM, cells were incubated with fiducial markers (Fokkema et al., 2018) at a concentration of 1 μg/ml and cell-permeable lysosome stain SiR-Lysosome (SpiroChrome) at a concentration of 0.5 μM in complete DMEM and incubated for 3 h.



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    construct encoding memerald sec61b c1  (Addgene inc)
    93
    Addgene inc construct encoding memerald sec61b c1
    Construct Encoding Memerald Sec61b C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/construct+encoding+memerald+sec61b+c1/mEmerald-Sec61b-C1+(Plasmid+%2390992)/pmc09035751-46-13-24
    Average 93 stars, based on 1 article reviews
    construct encoding memerald sec61b c1 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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